WebAdd 200 μL (or appropriate volume) of staining solution onto the biofilm sample. Add the stain very gently so as not to disturb the biofilm. It is important to immediately add the … WebS. mutans was cultivated in the presence of CBG, and the resulting biofilms were examined by CV staining, MTT assay, qPCR, biofilm tracer, optical profilometry, and SEM.

Filmtracer Live Dead Biofilm Viability Kit Thermo Fisher Bioz

WebMar 1, 2024 · Biofilms were washed with PBS, stained with 0.1% crystal violet, and assessed by OD 600nm on a plate reader. Biofilms were also stained with Ruby Red Biofilm Tracer (Thermo Fisher Scientific) and imaged by microscopy. Biofilms were quantified with Fiji software (National Institutes of Health) by tabulating the mean pixel … WebNov 20, 2024 · Biofilms were washed with PBS, stained with 0.1% crystal violet, and assessed by OD 600nm on a plate reader. Biofilms were also stained with Ruby Red Biofilm Tracer (Thermo Fisher Scientific) and … california bill sb 871

Transcriptomic analysis of Propionibacterium acnes

WebThe removal of phosphorus (P) from wastewater is important to reduce water pollution and potential eutrophication in surface waters. In previous studi… WebA variety of FilmTracer stains is available for imaging different components of a biofilm. The FilmTracer FM 1-43 dye (Figure 1) stains the cells in a biofilm, whereas FilmTracer … coach shoes made in china