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A custom insert was constructed as follows. Two BRAND Inserts for 12-well plates PET-membrane 0.4 µm (BrandTech Scientific Inc., CAT# 782730, Essex, CT, USA) were used to construct a “3D-Flipwell” (Figs. 2, 3). All work was performed under sterile conditions inside a cell culture cabinet (Class II A/B3 Biological … See more Caco-2 human colon epithelial cells were obtained from American Tissue Culture Collection (ATCC, CAT#: HTB-37, Manassas, VA). THP … See more The following reagents were used. l-Sepiapterin (SEP, Career Henan Chemical Co., CAS#: 17094-01-8, National University Science Park, High-Tech Zone, Zhengzhou City, … See more THP-1 cells were maintained at a cell density of 1 × 105/mL to 1 × 106/mL in RPMI 1640 (ThermoFisher Scientific, CAT#: 11875119, Waltham, MA) supplemented with 10% FBS, 1% GlutaMax … See more The following antibodies were used. Anti-human MUC2 (996/1) (ThermoFisher, CAT# MA5-12345); Anti-human CD68 (Novus Biologicals, CAT# NB100-683-0.1 mg, Centennial, CO, USA); Anti-human CK20 (Life … See more Web1 day ago · To be classified as an extreme El Niño, temperatures must be at least 2C higher. There’s still plenty of time before August, though, so researchers did warn against taking the climate models as ... simplicity boys shirt patterns
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