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Nphs2-cre

Web19 nov. 2002 · Twenty- four transgenic founders were generated in which Cre recombinase was placed under the regulation of a 2.5-kb fragment of the human NPHS2 promoter. Previously, this fragment was shown to drive beta-galactosidase (β-gal) expression exclusively in podocytes of transgenic mice. Web008205 B6.Cg-Tg(NPHS2-cre)295Lbh/J These transgenic mice (commonly referred to as podocin-Cre mice) have expression of Cre recombinase directed to podocytes within kidney glomeruli by the human podocin ( NPHS2 ) promoter/enhancer region and may be useful in generating Cre-lox conditional knockouts for studying the role of podocyte nephrobiology …

008205 - 2.5P-Cre Strain Details

WebTools. Data Hub. Learn more about Addgene materials from user-contributed reports describing AAV and antibody experiments. Sequence Analyzer. Basic analysis for a user-entered sequence; includes restriction sites and map Web21 mrt. 2024 · NPHS2 (NPHS2 Stomatin Family Member, Podocin) is a Protein Coding gene. Diseases associated with NPHS2 include Nephrotic Syndrome, Type 2 and Nephrotic Syndrome . Among its related pathways are Cell junction organization and Nephrin family interactions . An important paralog of this gene is STOM. UniProtKB/Swiss-Prot … columbia county ga tax collector https://drogueriaelexito.com

肾脏足细胞特异敲除lncRNADLX6-os1转基因小鼠的培育方法与流 …

Web本模型可利用Cre/loxP位点特异性重组系统对细胞命运进行追踪展示，揭示特定类型细胞在发育、疾病中的再生、自我更新、分化过程。 双荧光报告基因系统提供zsGreene及tdTomato两种荧光标记，实现了重组/非重组细胞的实时检测，因此，本模型也是一种示踪特异性Cre表达位置的理想工具。 图二 B6-G/R与Cre小鼠交配后部分组织荧光表达检测 好了~以上只是 … WebFull Sequences from Addgene (1) Based on next-generation sequencing (NGS) results where indicated (Addgene NGS Result), or assembled from reference sequences and/or Sanger results (Addgene Assembled Sequence). Analyze Sequence. GenBank. WebNPHS2-cre. 2024-11-19. B6.Cg-Tg(NPHS2-cre)295Lbh. 品系编号：GAP1021. 品系简称：NPHS2-cre. 品系特点：. NPHS2-Cre 小鼠可存活且可生育，Cre 重组酶的表达通过人 … columbia county ga school calendar 2019 20

乘风破浪的大鼠——如何突破技术限制再次发挥在特定领域的研究 …

Category:【双荧光报告基因小鼠】织出生物学天空最美丽的彩虹 - 知乎

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Nphs2-cre

NPHS2-Cre transgene efficiently deleted Dicer floxed region, …

Web23 aug. 2024 · 肾脏病防治转化医学研究中心成立于2024年，研究中心由三个实验室组成：干细胞与肾脏再生实验室、紧密连接功能与疾病实验室、泌尿系肿瘤实验室。. 实验室以“开放、流动、联合、竞争”八字方针为指导，坚持以基础研究为主，将本实验室的多学科特色与 ... Web9 sep. 2024 · The NPHS2-Cre transgene comprises the Cre coding sequence under the control of 2.5 kb of the human NPHS2 promoter. The NPHS2 gene encodes Podocin, which is specifically expressed in podocytes. In their paper, Balkawade et al. observed a thickening of the glomerular basement membrane (GBM) in 24-week-old NPHS2-Cre mice, which …

Nphs2-cre

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WebNPHS2-Cre transgene efficiently deleted Dicer floxed region, leading to downregulation of mature miRNA and accumulation of precursor miRNA in podocytes of conditional … Web12 nov. 2024 · cre工具鼠详细介绍 / 开普饭. cre小鼠是什么？. cre工具鼠详细介绍. 研究人员使用基因编辑的小鼠模型来研究基因表达，研究遗传疾病，进行临床前药物药效测试等。. 在许多研究中，小鼠模型在其整个发育和生命过程中均表达基因突变。. 然而，在某些研究中 ...

Web19 mrt. 2024 · For rapamycin treatment, Nphs2-Cre ( n = 17), Tsc2flox/flox ( n = 16), and Tsc2Δpodocyte ( n = 8) mice were intraperitoneally injected with rapamycin (LC Laboratories, Woburn, MA) at 2 mg kg -1 of body weight every other day … Web10 dec. 2024 · 本发明涉及肾脏足细胞特异敲除lncRNA DLX6‑os1转基因小鼠的培育方法，可有效解决在不同肾脏疾病中研究足细胞损伤的作用机制的问题，其解决的技术方案是，通过基因工程技术对前期建立的携带FLOX‑DLX6‑os1转基因小鼠与另一种携带足细胞特异的NPHS2‑Cre基因的 ...

Web实验小鼠是目前应用最为广泛的一种实验动物，在探寻基因基础功能，疾病发病机制以及药物临床前筛选等方面有着十分重要的作用。 其原因在于小鼠和人的基因具有极高的相似度（小鼠99%的基因能在人类基因组中找到同源… http://www.labmodel.cn/mod_product-view-p_id-4397.html

Web20 aug. 2015 · Next, we asked whether the protection from podocyte loss in diabetic NPHS2 cre Notch1 F/F mice could be related to the effect of Notch1 on podocyte apoptosis. We tested this by quantifying caspase 3/7 positive primary podocytes isolated from glomeruli of control, NPHS2 cre Notch1 F/F, and NPHS2 cre Notch2 F/F mice.

Webd, Relative mRNA amount of Fn1, Col1a1 and Col3a1 in control and Ksp cre /Dab2 flox/+ mice with or without FA injection. ... Tory K, et al. Mutation-dependent recessive inheritance of NPHS2-associated steroid-resistant nephrotic syndrome. Nat Genet 46, 299–304 (2014). columbia county ga title officeWeb7 sep. 2024 · The CD16/7-nephrin activation model allows for precisely-timed induction of nephrin tyrosine phosphorylation in live cells by addition of specific antibodies that cluster the extracellular CD16 domain and results in intracellular tyrosine phosphorylation of nephrin’s cytoplasmic tail. dr thomas hocevarWebPartial Sequences from Addgene (3) Addgene has sequenced portions of this plasmid for verification. The results are shown below. Leading primers are indicated on the first line of each sequence. Analyze Sequence. dr. thomas hoang urologyWeb3 sep. 2024 · We recently reported that the podocyte-specific knockdown of Klf4 using the Cre recombinase system (Klf4 ∆Pod) triggered podocyte loss, pathologic glomerular epithelial cell proliferation, and FSGS, leading to kidney dysfunction and death as compared to control (Klf4 fl/fl) mice ().While immunostaining revealed a loss of podocyte markers … columbia county ga state representativeWeb20 mrt. 2024 · NPHS2 Cre PAR-1 Active+/− mice were also in renal failure as evidenced by their significantly higher levels of serum creatinine and urea compared with littermate controls (Figure 4 c and d, respectively). Furthermore, NPHS2 Cre PAR-1 Active+/− mice died between the ages of 39 and 45 days (Figure 4 e). dr thomas hobby valdosta gaWebTwenty- four transgenic founders were generated in which Cre recombinase was placed under the regulation of a 2.5-kb fragment of the human NPHS2 promoter. Previously, this … dr thomas hoch zahnarztWebCre 蛋白是一种重组酶，是causes recombination的缩写，于1981 年从 P1 噬菌体中被发现，属于 λInt 酶超基因家族。 Cre 重组酶基因编码区序列全长1029bp，编码 38kDa 蛋白 … dr thomas hochholzer